Journal: International Journal of Molecular Sciences
Article Title: TGF-β Inhibition Through Combinatory Strategies Suppresses Proliferation and Invasiveness in Malignant Pleural Mesothelioma
doi: 10.3390/ijms27052157
Figure Lengend Snippet: Effect of TGF-β silencing on MPM cell migration and cell cycle. ( A ) Scratch assay evaluated on MSTO-211H cells after 48 h without (-) or with 60 nM TGF-β siRNA. Cells were analyzed by microscopy at the time of scratching (T0) and after 24 h (T1). Relative migration was calculated by setting the percentage migration of control cells at T1 equal to 100% and comparing the percentage migration of cells after transfection with siTGF-β to this value. Data are presented as means ± SD. **** p < 0.0001: cells transfected with siTGF-β vs. Ctrl cells. ( B ) Cell cycle analysis using flow cytometry after staining with propidium iodide (PI). ( C ) Distribution of cells in sub-G1, G1/G0, S, and G2/M phases of the cell cycle under different experimental conditions. Data are presented as means ± SD ( n = 3). **** p < 0.0001, ** p < 0.01 and * p < 0.05: treated cells vs. respective untreated cells (Ctrl).
Article Snippet: Experiments were performed on the commercially available MPM cell line MSTO-211H (human biphasic mesothelioma cells), purchased from American Type Culture Collection (ATCC; Manassas, VA, USA), and primary MPM patient-derived cell lines MM404 (human epithelioid histotype), MM421 (human biphasic histotype) and MM432 (human sarcomatoid histotype) from Biological Bank of Mesothelioma (SS.
Techniques: Migration, Wound Healing Assay, Microscopy, Control, Transfection, Cell Cycle Assay, Flow Cytometry, Staining